Enzymatic hydrolysis of organic-core microcapsules to produce aqueous-core microcapsules
This paper describes the development of a new method to obtain aqueous-core microcapsules from organic-core capsules. The direct production of microcapsules, using tripropionin as organic material, followed by the hydrolysis of the core by a lipase was investigated. The enzymatic study showed that the enzyme obeyed a Michaelis-Menten mechanism and conditions for optimal activity were pH 7.5, 25-37 degrees C and 0% NaCl. Under these conditions, incubation of tripropionin-alginate microcapsules in a buffer containing the enzyme successfully produced aqueous-core capsules with reduced accumulation of alginate in the core in similar to 3 h.
Keywords: alginate ; lipase ; tripropionin ; liquid-core capsules ; Lipase-Catalyzed Hydrolysis ; Candida-Rugosa Lipase ; Alginate Polycation ; Cell-Growth ; Fatty-Acids ; Microencapsulation ; Kinetics ; Culture ; System ; Transplantation
Record created on 2010-11-24, modified on 2016-08-08