000155429 001__ 155429
000155429 005__ 20180128024605.0
000155429 037__ $$aSTUDENT
000155429 245__ $$aModeling and analysis of ethanol production in Escherichia coli
000155429 260__ $$c2010
000155429 269__ $$a2010
000155429 336__ $$aStudent Projects
000155429 500__ $$aLaboratory of Computational Systems Biotechnology, EPFL
000155429 520__ $$aThe ethanol production for biofuels is meant to be increased in the next years, but the yield could be improved. The goal of this study is to build a model of Escherichia coli KO11 to perform a rational design to improve the yield of ethanol production. The model was written in Cell Designer. The kinetic equations were picked up from the literature, with their kinetic parameters. All parameters of the equations couldn’t be directly extracted from the literature, and the initial metabolite concentrations couldn’t be found in the literature, due to thermodynamical infeasibility or incompleteness of data. Flux balance analysis was used to determine the steady state fluxes and a thermodynamical analysis determined the metabolites concentration. Together these data allows computations of missing paramaters. The metabolite time course was governed by mass-balance equations and the system could be solved numerically. The main results are the enzyme maximal rates and the thermodynamically feasible initial concentrations. The next step was to correct the model for stability. Differents ways were explored to obtain stability of the model, but they failed
000155429 6531_ $$aFSV/SSV
000155429 6531_ $$aSciences et technologie du vivant
000155429 6531_ $$aLife sciences and technology
000155429 6531_ $$aCentral carbon metabolism
000155429 6531_ $$aStability
000155429 6531_ $$aKinetic modeling
000155429 6531_ $$aThermodynamic feasibility
000155429 6531_ $$a Escherichia coli
000155429 700__ $$aMettraux, Yannick
000155429 720_2 $$aHatzimanikatis, Vassily$$edir.
000155429 909C0 $$0252289$$pSSV
000155429 909CO $$ooai:infoscience.tind.io:155429$$pSV
000155429 917Z8 $$x182396
000155429 937__ $$aEPFL-STUDENT-155429
000155429 973__ $$aEPFL$$sPUBLISHED
000155429 980__ $$aSTUDENT$$bMASTERS