000154705 001__ 154705
000154705 005__ 20190416055745.0
000154705 0247_ $$2doi$$a10.1093/nar/gkp116
000154705 022__ $$a1362-4962
000154705 02470 $$2ISI$$a000265953000018
000154705 037__ $$aARTICLE
000154705 245__ $$aNucleosome eviction from MHC class II promoters controls positioning of the transcription start site
000154705 269__ $$a2009
000154705 260__ $$bOxford University Press$$c2009
000154705 336__ $$aJournal Articles
000154705 520__ $$aNucleosome depletion at transcription start sites (TSS) has been documented genome-wide in multiple eukaryotic organisms. However, the mechanisms that mediate this nucleosome depletion and its functional impact on transcription remain largely unknown. We have studied these issues at human MHC class II (MHCII) genes. Activation-induced nucleosome free regions (NFR) encompassing the TSS were observed at all MHCII genes. Nucleosome depletion was exceptionally strong, attaining over 250-fold, at the promoter of the prototypical HLA-DRA gene. The NFR was induced primarily by the transcription factor complex that assembles on the conserved promoter-proximal enhancer situated upstream of the TSS. Functional analyses performed in the context of native chromatin demonstrated that displacing the NFR without altering the sequence of the core promoter induced a shift in the position of the TSS. The NFR thus appears to play a critical role in transcription initiation because it directs correct TSS positioning in vivo. Our results provide support for a novel mechanism in transcription initiation whereby the position of the TSS is controlled by nucleosome eviction rather than by promoter sequence.
000154705 6531_ $$aGenes
000154705 6531_ $$aMHC Class II
000154705 6531_ $$aPromoter Regions
000154705 6531_ $$aGenetic
000154705 6531_ $$aTranscription Initiation Site
000154705 700__ $$aLeimgruber, Elisa
000154705 700__ $$aSeguín-Estévez, Queralt
000154705 700__ $$aDunand-Sauthier, Isabelle
000154705 700__ $$aRybtsova, Natalia
000154705 700__ $$aSchmid, Christoph D.
000154705 700__ $$0243605$$aAmbrosini, Giovanna$$g176602
000154705 700__ $$0244404$$aBucher, Philipp$$g113607
000154705 700__ $$aReith, Walter
000154705 773__ $$j37$$k8$$q2514-28$$tNucleic acids research
000154705 8564_ $$s1024309$$uhttps://infoscience.epfl.ch/record/154705/files/Nucl.%20Acids%20Res.-2009-Leimgruber-2514-28.pdf$$yPublisher's version$$zPublisher's version
000154705 909C0 $$0252244$$pGR-BUCHER$$xU11780
000154705 909CO $$ooai:infoscience.tind.io:154705$$pSV$$particle$$qGLOBAL_SET
000154705 917Z8 $$x182396
000154705 917Z8 $$x182396
000154705 937__ $$aEPFL-ARTICLE-154705
000154705 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000154705 980__ $$aARTICLE