A Ca2+ ion selective electrode biosensor in microfluidics to monitor hepatocyte’s activities

Ca2+ plays a crucial role in cell communication. For example, periodical changes in Ca2+ concentration act as cell sig- nals. Intracellular Ca2+ signals have been measured by Ca2+ sensitive fluorescent dye [1]. In this study, extracellular Ca2+ variation was monitored by a potentiometry using a solid-state type of Ca2+ Ion Selective Electrode (ISE). Ca2+ ISE had high sensitivity detecting down to 10-10M of calcium concentration, as well as high selectivity against K+. After KCl sti- mulus of cells in a microchannel, we observed an oscillating increase in Ca2+ concentration, associated with a periodic oscillation of cell activity (Hepatocyte, Hep2G).!


Published in:
Proceedings of the 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), 1, 1, 247-249
Presented at:
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010), Groningen, The Netherlands, 3-7 october 2010
Year:
2010
ISBN:
978-0-9798064-3-8
Keywords:
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 Record created 2010-11-02, last modified 2018-03-17

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