000151370 001__ 151370
000151370 005__ 20181203022000.0
000151370 022__ $$a0026-8925
000151370 02470 $$2PMID$$a3892228
000151370 0247_ $$2doi$$a10.1007/BF00332940
000151370 037__ $$aARTICLE
000151370 245__ $$aMolecular characterisation of the colicin E2 operon and identification of its products
000151370 269__ $$a1985
000151370 260__ $$c1985
000151370 336__ $$aJournal Articles
000151370 520__ $$aThe DNA sequence of the entire colicin E2 operon was determined. The operon comprises the colicin activity gene, ceaB, the colicin immunity gene, ceiB, and the lysis gene, celB, which is essential for colicin release from producing cells. A potential LexA binding site is located immediately upstream from ceaB, and a rho-independent terminator structure is located immediately downstream from celB. A comparison of the predicted amino acid sequences of colicin E2 and cloacin DF13 revealed extensive stretches of homology. These colicins have different modes of action and recognise different cell surface receptors; the two major regions of heterology at the carboxy terminus, and in the carboxy-terminal end of the central region probably correspond to the catalytic and receptor-recognition domains, respectively. Sequence homologies between colicins E2, A and E1 were less striking, and the colicin E2 immunity protein was not found to share extensive homology with the colicin E3 or cloacin DF13 immunity proteins. The lysis proteins of the ColE2, ColE1 and CloDF13 plasmids are almost identical except in the aminoterminal regions, which themselves have overall similarity with lipoprotein signal peptides. Processing of the ColE2 prolysis protein to the mature form was prevented by globomycin, a specific inhibitor of the lipoprotein signal peptidase. The mature ColE2 lysis protein was located in the cell envelope. The results are discussed in terms of the functional organisation of the colicin operons and the colicin proteins, and the way in which colicins are released from producing cells.
000151370 6531_ $$aBacteriocin Plasmids
000151370 6531_ $$aPlasmids
000151370 700__ $$0243892$$g177247$$aCole, S T
000151370 700__ $$aSaint-Joanis, B
000151370 700__ $$aPugsley, A P
000151370 773__ $$j198$$tMolecular & general genetics : MGG$$k3$$q465-72
000151370 909C0 $$xU11742$$0252302$$pUPCOL
000151370 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151370
000151370 937__ $$aEPFL-ARTICLE-151370
000151370 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151370 980__ $$aARTICLE