000151344 001__ 151344
000151344 005__ 20181203022000.0
000151344 022__ $$a0026-8925
000151344 02470 $$2PMID$$a2560137
000151344 0247_ $$2doi$$a10.1007/BF00259619
000151344 037__ $$aARTICLE
000151344 245__ $$aGene cloning shows the alpha-toxin of Clostridium perfringens to contain both sphingomyelinase and lecithinase activities
000151344 269__ $$a1989
000151344 260__ $$c1989
000151344 336__ $$aJournal Articles
000151344 520__ $$aThe plc gene encoding the alpha-toxin (phospholipase C), an important virulence factor of Clostridium perfringens, has been cloned, sequenced and expressed in Escherichia coli. Transcriptional analysis of mRNAs produced in vivo by C. perfringens and E. coli, and in vitro using purified RNA polymerase from C. perfringens revealed that plc is transcribed constitutively from a single promoter situated about 100 nucleotides from the coding sequence. A T7 expression system was used to overproduce alpha-toxin in E. coli; enzymological studies with the amplified plc gene product unambiguously demonstrated that both lecithinase (phospholipase C) and sphingomyelinase activities were associated with this 43,000 dalton cytotoxin. The 370-residue alpha-toxin is haemolytic and shares sequence and functional homology with the two components of Bacillus cereus haemolysin, cereolysin AB, in which phospholipase C and sphingomyelinase activities are associated with different polypeptides.
000151344 6531_ $$aCalcium-Binding Proteins
000151344 6531_ $$aType C Phospholipases
000151344 700__ $$aSaint-Joanis, B
000151344 700__ $$aGarnier, T
000151344 700__ $$g177247$$aCole, S T$$0243892
000151344 773__ $$j219$$tMolecular & general genetics : MGG$$k3$$q453-60
000151344 909C0 $$xU11742$$0252302$$pUPCOL
000151344 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151344
000151344 937__ $$aEPFL-ARTICLE-151344
000151344 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151344 980__ $$aARTICLE