000151337 001__ 151337
000151337 005__ 20181203022000.0
000151337 022__ $$a0026-8925
000151337 02470 $$2PMID$$a1851953
000151337 0247_ $$2doi$$a10.1007/BF00273621
000151337 037__ $$aARTICLE
000151337 245__ $$aCharacterization of two genes, glpQ and ugpQ, encoding glycerophosphoryl diester phosphodiesterases of Escherichia coli
000151337 269__ $$a1991
000151337 260__ $$c1991
000151337 336__ $$aJournal Articles
000151337 520__ $$aThe nucleotide sequences of the glpQ and ugpQ genes of Escherichia coli, which both encode glycerophosphoryl diester phosphodiesterases, were determined. The glpQ gene encodes a periplasmic enzyme of 333 amino acids, produced initially with a 25 residue long signal sequence, while ugpQ codes for a cytoplasmic protein of 247 amino acids. Despite differences in size and cellular location, significant similarity in the primary structures of the two enzymes was found suggesting a common evolutionary origin. The 3' end of the ugpQ gene overlaps an open reading frame that is transcribed in the opposite direction. This open reading frame encodes a polypeptide with an unusual composition, i.e., 46 of the 146 amino acids are Gln or Asn. This polypeptide and the UgpQ protein were identified in an in vitro transcription/translation system as proteins with apparent molecular weights of 19.5 and 27 kDa, respectively.
000151337 6531_ $$aGenes, Bacterial
000151337 700__ $$aTommassen, J
000151337 700__ $$aEiglmeier, K
000151337 700__ $$0243892$$g177247$$aCole, S T
000151337 700__ $$aOverduin, P
000151337 700__ $$aLarson, T J
000151337 700__ $$aBoos, W
000151337 773__ $$j226$$tMolecular & general genetics : MGG$$k1-2$$q321-7
000151337 909C0 $$xU11742$$0252302$$pUPCOL
000151337 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151337
000151337 937__ $$aEPFL-ARTICLE-151337
000151337 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151337 980__ $$aARTICLE