000151305 001__ 151305
000151305 005__ 20181203021959.0
000151305 022__ $$a0950-382X
000151305 02470 $$2PMID$$a8733228
000151305 0247_ $$2doi$$a10.1111/j.1365-2958.1996.tb02617.x
000151305 037__ $$aARTICLE
000151305 245__ $$aOrganization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis
000151305 269__ $$a1996
000151305 260__ $$c1996
000151305 336__ $$aJournal Articles
000151305 520__ $$aThe genus Mycobacterium is composed of species with widely differing growth rates ranging from approximately three hours in Mycobacterium smegmatis to two weeks in Mycobacterium leprae. As DNA replication is coupled to cell duplication, it may be regulated by common mechanisms. The chromosomal regions surrounding the origins of DNA replication from M. smegmatis, M. tuberculosis, and M. leprae have been sequenced, and show very few differences. The gene order, rnpA-rpmH-dnaA-dnaN-recF-orf-gyrB-gyrA, is the same as in other Gram-positive organisms. Although the general organization in M. smegmatis is very similar to that of Streptomyces spp., a closely related genus, M. tuberculosis and M. leprae differ as they lack an open reading frame, between dnaN and recF, which is similar to the gnd gene of Escherichia coli. Within the three mycobacterial species, there is extensive sequence conservation in the intergenic regions flanking dnaA, but more variation from the consensus DnaA box sequence was seen than in other bacteria. By means of subcloning experiments, the putative chromosomal origin of replication of M. smegmatis, containing the dnaA-dnaN region, was shown to promote autonomous replication in M. smegmatis, unlike the corresponding regions from M. tuberculosis or M. leprae.
000151305 6531_ $$aReplication Origin
000151305 700__ $$aSalazar, L
000151305 700__ $$aFsihi, H
000151305 700__ $$ade Rossi, E
000151305 700__ $$aRiccardi, G
000151305 700__ $$aRios, C
000151305 700__ $$0243892$$aCole, S T$$g177247
000151305 700__ $$aTakiff, H E
000151305 773__ $$j20$$k2$$q283-93$$tMolecular microbiology
000151305 909C0 $$0252302$$pUPCOL$$xU11742
000151305 909CO $$ooai:infoscience.tind.io:151305$$pSV$$particle
000151305 937__ $$aEPFL-ARTICLE-151305
000151305 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000151305 980__ $$aARTICLE