000151304 001__ 151304
000151304 005__ 20181203021959.0
000151304 022__ $$a0027-8424
000151304 02470 $$2PMID$$a8610181
000151304 0247_ $$2doi$$a10.1073/pnas.93.7.3132
000151304 037__ $$aARTICLE
000151304 245__ $$aAn integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae
000151304 269__ $$a1996
000151304 260__ $$c1996
000151304 336__ $$aJournal Articles
000151304 520__ $$aAn integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.
000151304 6531_ $$aChromosomes, Bacterial
000151304 6531_ $$aGenome, Bacterial
000151304 700__ $$aPhilipp, W J
000151304 700__ $$aPoulet, S
000151304 700__ $$aEiglmeier, K
000151304 700__ $$aPascopella, L
000151304 700__ $$aBalasubramanian, V
000151304 700__ $$aHeym, B
000151304 700__ $$aBergh, S
000151304 700__ $$aBloom, B R
000151304 700__ $$aJacobs, W R
000151304 700__ $$0243892$$aCole, S T$$g177247
000151304 773__ $$j93$$k7$$q3132-7$$tProceedings of the National Academy of Sciences of the United States of America
000151304 909C0 $$0252302$$pUPCOL$$xU11742
000151304 909CO $$ooai:infoscience.tind.io:151304$$pSV$$particle
000151304 937__ $$aEPFL-ARTICLE-151304
000151304 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000151304 980__ $$aARTICLE