000151253 001__ 151253
000151253 005__ 20181203021958.0
000151253 0247_ $$2doi$$a10.1046/j.1365-2958.2002.02813.x
000151253 022__ $$a0950-382X
000151253 02470 $$2PMID$$a11967065
000151253 037__ $$aARTICLE
000151253 245__ $$aAre the PE-PGRS proteins of Mycobacterium tuberculosis variable surface antigens?
000151253 269__ $$a2002
000151253 260__ $$c2002
000151253 336__ $$aJournal Articles
000151253 520__ $$aMycobacterium tuberculosis H37Rv contains 67 PE-PGRS genes, with multiple tandem repetitive sequences, encoding closely related proteins that are exceptionally rich in glycine and alanine. As no functional information was available, 10 of these genes were selected and shown to be expressed in vitro by reverse transcription-polymerase chain reaction (RT-PCR). Antibodies against five PE-PGRS proteins, raised in mice by DNA vaccination, detected single proteins when the same plasmid constructs used for immunization were expressed in epithelial cells or in reticulocyte extracts, confirming that the PE-PGRS proteins are antigenic. As expected from the conserved repetitive structure, the antibodies cross-reacted with more than one PE-PGRS protein, suggesting that different proteins share common epitopes. PE-PGRS proteins were detected by West-ern blotting in five different mycobacterial species (M. tuberculosis, M. bovis BCG, M. smegmatis, M. marinum and M. gordonae) and 11 clinical isolates of M. tuberculosis. Whole-genome comparisons of M. tuberculosis predicted allelic diversity in the PE-PGRS family, and this was confirmed by immunoblot studies as size variants were detected in clinical strains. Subcellular fractionation studies and immunoelectron microscopy localized many PE-PGRS proteins in the cell wall and cell membrane of M. tuberculosis. The data suggest that some PE-PGRS proteins are variable surface antigens.
000151253 700__ $$aBanu, Sayera
000151253 700__ $$aHonoré, Nadine
000151253 700__ $$aSaint-Joanis, Brigitte
000151253 700__ $$aPhilpott, Dana
000151253 700__ $$aPrévost, Marie-Christine
000151253 700__ $$g177247$$aCole, Stewart T$$0243892
000151253 773__ $$j44$$tMolecular microbiology$$k1$$q9-19
000151253 909C0 $$xU11742$$0252302$$pUPCOL
000151253 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151253
000151253 937__ $$aEPFL-ARTICLE-151253
000151253 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151253 980__ $$aARTICLE