000151252 001__ 151252
000151252 005__ 20181203021958.0
000151252 022__ $$a0095-1137
000151252 02470 $$2PMID$$a12089245
000151252 0247_ $$2doi$$a10.1128/JCM.40.7.2339-2345.2002
000151252 037__ $$aARTICLE
000151252 245__ $$aRapid and simple approach for identification of Mycobacterium tuberculosis complex isolates by PCR-based genomic deletion analysis
000151252 269__ $$a2002
000151252 260__ $$c2002
000151252 336__ $$aJournal Articles
000151252 520__ $$aAlthough the virulences and host ranges differ among members of the Mycobacterium tuberculosis complex (TBC; M. tuberculosis, M. africanum, M. canettii, M. microti, M. bovis, and M. bovis BCG), commercially available molecular assays cannot differentiate these organisms because of the genetic identities of their 16S rRNA gene sequences. Comparative genomic analyses with the complete DNA sequence of M. tuberculosis H37Rv has provided information on regions of difference (RD 1 to RD 16) deleted in members of the TBC other than M. tuberculosis. To determine whether deletion analysis could accurately differentiate members of TBC, we used PCR to assess the presence or absence of specific regions of the genome in 88 well-characterized isolates of M. tuberculosis, M. africanum, M. microti, M. bovis, and M. bovis BCG. The identifications obtained by use of the specific deletion profiles correlated 100% with the original identifications for all TBC members except M. africanum, but further characterization resulted in profiles specific for all members. Although six RD regions were used in the analyses with the original 88 isolates, it was found that the use of RD 1, RD 9, and RD 10 was sufficient for initial screenings, followed by the use of RD 3, RD 5, and RD 11 if the results for any of the first three regions were negative. When 605 sequential clinical isolates were screened, 578 (96%) were identified as M. tuberculosis, 6 (1%) were identified as M. africanum, 8 (1%) were identified as M. bovis, and 13 (2%) were identified as M. bovis BCG. Since PCR-based assays can be implemented in most clinical mycobacteriology laboratories, this approach provides a rapid and simple means for the differentiation of members of TBC, especially M. bovis and M. tuberculosis, when it is important to distinguish between zoonotic sources (i.e., cattle and unpasteurized dairy products) and human sources of tuberculosis disease.
000151252 700__ $$aParsons, Linda M
000151252 700__ $$aBrosch, Roland
000151252 700__ $$0243892$$g177247$$aCole, Stewart T
000151252 700__ $$aSomoskövi, Akos
000151252 700__ $$aLoder, Arthur
000151252 700__ $$aBretzel, Gisela
000151252 700__ $$aVan Soolingen, Dick
000151252 700__ $$aHale, Yvonne M
000151252 700__ $$aSalfinger, Max
000151252 773__ $$j40$$tJournal of clinical microbiology$$k7$$q2339-45
000151252 909C0 $$xU11742$$0252302$$pUPCOL
000151252 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151252
000151252 937__ $$aEPFL-ARTICLE-151252
000151252 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151252 980__ $$aARTICLE