000151250 001__ 151250
000151250 005__ 20181203021958.0
000151250 022__ $$a0019-9567
000151250 02470 $$2PMID$$a12228284
000151250 0247_ $$2doi$$a10.1128/IAI.70.10.5568-5578.2002
000151250 037__ $$aARTICLE
000151250 245__ $$aBacterial artificial chromosome-based comparative genomic analysis identifies Mycobacterium microti as a natural ESAT-6 deletion mutant
000151250 269__ $$a2002
000151250 260__ $$c2002
000151250 336__ $$aJournal Articles
000151250 520__ $$aMycobacterium microti is a member of the Mycobacterium tuberculosis complex that causes tuberculosis in voles. Most strains of M. microti are harmless for humans, and some have been successfully used as live tuberculosis vaccines. In an attempt to identify putative virulence factors of the tubercle bacilli, genes that are absent from the avirulent M. microti but present in human pathogen M. tuberculosis or Mycobacterium bovis were searched for. A minimal set of 50 bacterial artificial chromosome (BAC) clones that covers almost all of the genome of M. microti OV254 was constructed, and individual BACs were compared to the corresponding BACs from M. bovis AF2122/97 and M. tuberculosis H37Rv. Comparison of pulsed-field gel-separated DNA digests of BAC clones led to the identification of 10 regions of difference (RD) between M. microti OV254 and M. tuberculosis. A 14-kb chromosomal region (RD1(mic)) that partly overlaps the RD1 deletion in the BCG vaccine strain was missing from the genomes of all nine tested M. microti strains. This region covers 13 genes, Rv3864 to Rv3876, in M. tuberculosis, including those encoding the potent ESAT-6 and CFP-10 antigens. In contrast, RD5(mic), a region that contains three phospholipase C genes (plcA to -C), was missing from only the vole isolates and was present in M. microti strains isolated from humans. Apart from RD1(mic) and RD5(mic) other M. microti-specific deleted regions have been identified (MiD1 to MiD3). Deletion of MiD1 has removed parts of the direct repeat region in M. microti and thus contributes to the characteristic spoligotype of M. microti strains.
000151250 6531_ $$aGenome, Bacterial
000151250 700__ $$aBrodin, Priscille
000151250 700__ $$aEiglmeier, Karin
000151250 700__ $$aMarmiesse, Magali
000151250 700__ $$aBillault, Alain
000151250 700__ $$aGarnier, Thierry
000151250 700__ $$aNiemann, Stefan
000151250 700__ $$0243892$$g177247$$aCole, Stewart T
000151250 700__ $$aBrosch, Roland
000151250 773__ $$j70$$tInfection and immunity$$k10$$q5568-78
000151250 909C0 $$xU11742$$0252302$$pUPCOL
000151250 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151250
000151250 937__ $$aEPFL-ARTICLE-151250
000151250 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000151250 980__ $$aARTICLE