000151237 001__ 151237
000151237 005__ 20181203021958.0
000151237 022__ $$a0019-9567
000151237 02470 $$2PMID$$a15155617
000151237 0247_ $$2doi$$a10.1128/IAI.72.6.3161-3170.2004
000151237 037__ $$aARTICLE
000151237 245__ $$aComparative analysis of B- and T-cell epitopes of Mycobacterium leprae and Mycobacterium tuberculosis culture filtrate protein 10
000151237 269__ $$a2004
000151237 260__ $$c2004
000151237 336__ $$aJournal Articles
000151237 520__ $$aCulture filtrate protein 10 (CFP-10) from Mycobacterium tuberculosis is a well-characterized immunodominant 10-kDa protein antigen known to elicit a very potent early gamma interferon response in T cells from M. tuberculosis-infected mice and humans. The sequence of the Mycobacterium leprae homologue of CFP-10 shows only 40% identity (60% homology) at the protein level with M. tuberculosis CFP-10 and thus has the potential for development as a T- or B-cell reactive antigen for specific diagnosis of leprosy. Antisera raised in mice or rabbits against recombinant M. leprae and M. tuberculosis CFP-10 proteins reacted only with homologous peptides from arrays of overlapping synthetic peptides, indicating that there was no detectable cross-reactivity at the antibody level. Sera from leprosy and tuberculosis patients were also specific for the homologous protein or peptides and showed distinct patterns of recognition for either M. leprae or M. tuberculosis CFP-10 peptides. At the cellular level, only 2 of 45 mouse T-cell hybridomas raised against either M. leprae or M. tuberculosis CFP-10 displayed a cross-reactive response against the N-terminal heterologous CFP-10 peptide, the region that exhibits the highest level of identity in the two proteins; however, the majority of peptide epitopes recognized by mouse T-cell hybridomas specific for each protein did not cross-react with heterologous peptides. Coupled with the human serology data, these results raise the possibility that peptides that could be used to differentiate infections caused by these two related microorganisms could be developed. Immunohistochemical staining of sections of M. leprae-infected nude mouse footpads resulted in strongly positive staining in macrophages and dendritic cells, as well as weaker staining in extracellular areas, suggesting that M. leprae CFP-10, like its homologue in M. tuberculosis, is a secreted protein.
000151237 700__ $$aSpencer, John S
000151237 700__ $$aKim, Hee Jin
000151237 700__ $$aMarques, Angela M
000151237 700__ $$aGonzalez-Juarerro, Mercedes
000151237 700__ $$aLima, Monica C B S
000151237 700__ $$aVissa, Varalakshmi D
000151237 700__ $$aTruman, Richard W
000151237 700__ $$aGennaro, Maria Laura
000151237 700__ $$aCho, Sang-Nae
000151237 700__ $$0243892$$aCole, Stewart T$$g177247
000151237 700__ $$aBrennan, Patrick J
000151237 773__ $$j72$$k6$$q3161-70$$tInfection and immunity
000151237 909C0 $$0252302$$pUPCOL$$xU11742
000151237 909CO $$ooai:infoscience.tind.io:151237$$pSV$$particle
000151237 937__ $$aEPFL-ARTICLE-151237
000151237 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000151237 980__ $$aARTICLE