000151213 001__ 151213
000151213 005__ 20180317093649.0
000151213 022__ $$a1286-4579
000151213 02470 $$2PMID$$a16797206
000151213 0247_ $$2doi$$a10.1016/j.micinf.2006.04.002
000151213 037__ $$aARTICLE
000151213 245__ $$aTowards an immunodiagnostic test for leprosy
000151213 269__ $$a2006
000151213 260__ $$c2006
000151213 336__ $$aJournal Articles
000151213 520__ $$aIn addition to multidrug therapy, elimination of leprosy requires improved diagnostic methods. Using a comparative genomics approach, 17 potential protein antigens (MLP) that are restricted to Mycobacterium leprae, or of limited distribution, were produced and tested for antigen-specific immune responses on leprosy patients, healthy contacts of leprosy patients, and tuberculosis patients in Mali and Bangladesh, as well as on non-endemic controls. T-cell antigenicity of MLP was confirmed by IFN-gamma production in whole-blood assays with the highest responses observed in paucibacillary leprosy patients and healthy contacts. Four MLP behaved well in both countries and induced significantly different responses between the study groups. Peptides carrying T cell epitopes from one of the antigens gave promising results in restimulation assays in mice and immune responses were not influenced by prior exposure to BCG or environmental mycobacteria. This study provides the immunological framework for the development of a specific, peptide-based immunodiagnostic test for leprosy.
000151213 700__ $$aAráoz, Romulo
000151213 700__ $$aHonoré, Nadine
000151213 700__ $$aBanu, Sayera
000151213 700__ $$aDemangel, Caroline
000151213 700__ $$aCissoko, Yakouba
000151213 700__ $$aArama, Charles
000151213 700__ $$aUddin, Mohammad Khaja Mafij
000151213 700__ $$aHadi, S K Abdul
000151213 700__ $$aMonot, Marc
000151213 700__ $$aCho, Sang-Nae
000151213 700__ $$aJi, Baohong
000151213 700__ $$aBrennan, Patrick J
000151213 700__ $$aSow, Samba
000151213 700__ $$0243892$$aCole, Stewart T$$g177247
000151213 773__ $$j8$$k8$$q2270-6$$tMicrobes and infection / Institut Pasteur
000151213 909CO $$ooai:infoscience.tind.io:151213$$particle$$pSV
000151213 909C0 $$0252302$$pUPCOL$$xU11742
000151213 937__ $$aEPFL-ARTICLE-151213
000151213 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000151213 980__ $$aARTICLE