000151192 001__ 151192
000151192 005__ 20190316234848.0
000151192 022__ $$a1615-9861
000151192 02470 $$2PMID$$a18615429
000151192 02470 $$a000258503400014$$2ISI
000151192 0247_ $$2doi$$a10.1002/pmic.200701018
000151192 037__ $$aARTICLE
000151192 245__ $$aComprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis
000151192 269__ $$a2008
000151192 260__ $$c2008
000151192 336__ $$aJournal Articles
000151192 520__ $$aMycobacterium ulcerans is the causative agent of Buruli ulcer, a rapidly emerging human disease in which mycolactone, a cytotoxic and immunosuppressive macrocyclic polyketide, is responsible for massive skin destruction. The genome sequencing of M. ulcerans has recently been accomplished (http://genolist.pasteur.fr/BuruList/) enabling the first proteome study of this important human pathogen. Here, we present a comprehensive proteome analysis of different subcellular fractions and culture supernatant of in vitro grown M. ulcerans. By a combination of gel-based and gel-free techniques for protein and peptide separation with subsequent analysis by MS, we identified 1074 different proteins, corresponding to 25% of the protein-coding DNA sequence. Interestingly, new information was obtained about central metabolism and lipid biosynthesis, and as many as 192 conserved hypothetical proteins were found. Comparative analysis of the wild-type strain and an isogenic mycolactone-deficient mutant, by 2-DE and iTRAQ labeling of the cytoplasmic fraction, revealed differences in the expression profiles of proteins involved in lipid metabolism and information pathways, as well as stress responses.
000151192 700__ $$aTafelmeyer, Petra
000151192 700__ $$aLaurent, Christine
000151192 700__ $$aLenormand, Pascal
000151192 700__ $$aRousselle, Jean-Claude
000151192 700__ $$aMarsollier, Laurent
000151192 700__ $$aReysset, Gilles
000151192 700__ $$aZhang, Runxuan
000151192 700__ $$aSickmann, Albert
000151192 700__ $$aStinear, Timothy P
000151192 700__ $$aNamane, Abdelkader
000151192 700__ $$g177247$$aCole, Stewart T$$0243892
000151192 773__ $$j8$$tProteomics$$k15$$q3124-38
000151192 909C0 $$xU11742$$0252302$$pUPCOL
000151192 909CO $$ooai:infoscience.tind.io:151192$$qGLOBAL_SET$$pSV$$particle
000151192 937__ $$aEPFL-ARTICLE-151192
000151192 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000151192 980__ $$aARTICLE