000151176 001__ 151176
000151176 005__ 20181203021956.0
000151176 022__ $$a1098-5514
000151176 02470 $$2PMID$$a20610710
000151176 0247_ $$2doi$$a10.1128/JVI.00771-10
000151176 037__ $$aARTICLE
000151176 245__ $$aApplication of broad-spectrum resequencing microarray for genotyping rhabdoviruses
000151176 269__ $$a2010
000151176 260__ $$c2010
000151176 336__ $$aJournal Articles
000151176 520__ $$aThe rapid and accurate identification of pathogens is critical in the control of infectious disease. To this end, we analyzed the capacity for viral detection and identification of a newly described high-density resequencing microarray (RMA), termed PathogenID, which was designed for multiple pathogen detection using database similarity searching. We focused on one of the largest and most diverse viral families described to date, the family Rhabdoviridae. We demonstrate that this approach has the potential to identify both known and related viruses for which precise sequence information is unavailable. In particular, we demonstrate that a strategy based on consensus sequence determination for analysis of RMA output data enabled successful detection of viruses exhibiting up to 26% nucleotide divergence with the closest sequence tiled on the array. Using clinical specimens obtained from rabid patients and animals, this method also shows a high species level concordance with standard reference assays, indicating that it is amenable for the development of diagnostic assays. Finally, 12 animal rhabdoviruses which were currently unclassified, unassigned, or assigned as tentative species within the family Rhabdoviridae were successfully detected. These new data allowed an unprecedented phylogenetic analysis of 106 rhabdoviruses and further suggest that the principles and methodology developed here may be used for the broad-spectrum surveillance and the broader-scale investigation of biodiversity in the viral world.
000151176 700__ $$aDacheux, Laurent
000151176 700__ $$aBerthet, Nicolas
000151176 700__ $$aDissard, Gabriel
000151176 700__ $$aHolmes, Edward C
000151176 700__ $$aDelmas, Olivier
000151176 700__ $$aLarrous, Florence
000151176 700__ $$aGuigon, Ghislaine
000151176 700__ $$aDickinson, Philip
000151176 700__ $$aFaye, Ousmane
000151176 700__ $$aSall, Amadou A
000151176 700__ $$aOld, Iain G
000151176 700__ $$aKong, Katherine
000151176 700__ $$aKennedy, Giulia C
000151176 700__ $$aManuguerra, Jean-Claude
000151176 700__ $$0243892$$g177247$$aCole, Stewart T
000151176 700__ $$aCaro, Valérie
000151176 700__ $$aGessain, Antoine
000151176 700__ $$aBourhy, Hervé
000151176 773__ $$j84$$tJournal of virology$$k18$$q9557-74
000151176 909C0 $$xU11742$$0252302$$pUPCOL
000151176 909CO $$pSV$$particle$$ooai:infoscience.tind.io:151176
000151176 937__ $$aEPFL-ARTICLE-151176
000151176 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000151176 980__ $$aARTICLE