000151174 001__ 151174
000151174 005__ 20181203021956.0
000151174 0247_ $$2doi$$a10.1111/j.1365-2958.2010.07313.x
000151174 022__ $$a1365-2958
000151174 02470 $$2PMID$$a20659288
000151174 02470 $$2ISI$$a000282172900007
000151174 037__ $$aARTICLE
000151174 245__ $$aTranscriptional regulation of lipid homeostasis in mycobacteria
000151174 260__ $$c2010
000151174 269__ $$a2010
000151174 336__ $$aJournal Articles
000151174 520__ $$aMycolic acids are major components of the cell envelope of mycobacteria, such as Mycobacterium tuberculosis, and play an important role in its architecture, impermeability and interaction with the environment. Synthesis of mycolic acids is carried out by two types of fatty acid synthases (FAS) working in concert: type I FAS, a multifunctional enzyme capable of de novo synthesis of medium-chain fatty acids, and type II FAS, responsible for their elongation. In this article we report the identification and characterization of a transcriptional regulator (MabR), whose binding to the FAS-II promoter region was demonstrated in vitro and in vivo. Overexpression and knock-down studies in Mycobacterium smegmatis revealed the repressor nature of MabR, with reduced amounts of FAS-II transcripts and fatty acids in the overproducing strain. Under these conditions, downregulation of fas transcription was also observed, thereby suggesting the existence of cross-talk between the two FAS, mediated by MabR. Finally, the finding that a mabR knock-out mutant could only be obtained in a merodiploid strain of M. smegmatis, confirmed the predicted essentiality, thus implying an essential role for MabR in mycobacterial fatty acid metabolism.
000151174 6531_ $$aFatty-Acid-Metabolism
000151174 6531_ $$aEscherichia-Coli
000151174 6531_ $$aMycolic Acids
000151174 6531_ $$aCondensing Enzymes
000151174 6531_ $$aGene-Expression
000151174 6531_ $$aAcyl-Coenzyme
000151174 6531_ $$aTuberculosis
000151174 6531_ $$aFadr
000151174 6531_ $$aSynthase
000151174 6531_ $$aBiosynthesis
000151174 700__ $$aSalzman, V
000151174 700__ $$aMondino, S
000151174 700__ $$0244366$$aSala, C$$g179660
000151174 700__ $$0243892$$aCole, S T$$g177247
000151174 700__ $$aGago, G
000151174 700__ $$aGramajo, H
000151174 773__ $$j78$$k1$$q64–77$$tMolecular microbiology
000151174 909C0 $$0252302$$pUPCOL$$xU11742
000151174 909CO $$ooai:infoscience.tind.io:151174$$pSV$$particle
000151174 917Z8 $$x148230
000151174 937__ $$aEPFL-ARTICLE-151174
000151174 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000151174 980__ $$aARTICLE