Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting

We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the electrode surface. The electrical field distribution is calculated in different electrode configurations by numerical simulations. Cell sorting shows high lysis efficiency, 99% of yeast cells sorted after lysis featuring dielectric properties similar to dead cells. A study of the potential device throughput is performed.


Published in:
Lab on a Chip, 10, 16, 2077-2082
Year:
2010
Publisher:
Royal Society of Chemistry
ISSN:
1473-0197
Laboratories:




 Record created 2010-09-07, last modified 2018-03-17

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