Triplet Imaging of Oxygen Consumption during the Contraction of a Single Smooth Muscle Cell (A7r5)

Triplet imaging is a novel optical technique that allows investigating oxy- gen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolution, which are required for the monitoring of fast kinetics of oxygen concentration in living cells. Calibration and validation is demon- strated with a titration experiment using L-Ascorbic Acid with the enzyme Ascor- base oxidase. The method was applied to a biological cell system, employing as reporter a cytosolic fusion protein of β-galactosidase with SNAP-tag labeled with tetramethylrhodamine. Oxygen consumption in single smooth muscle cells A7r5 during an [Arg8]-vasopressin-induced contraction is measured. The results indicate a consumption leading to an intracellular oxygen concentration that decays mono- exponentially with time. This is in good agreement with previously reported mea- surements of oxygen consumption in skeletal muscle fibers.


Presented at:
Conference of international society on oxygen transport to tissue, Monte Verità, Ascona, Switzerland, July 18-23, 2010
Year:
2010
Publisher:
Springer
Laboratories:




 Record created 2010-08-05, last modified 2018-03-18


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