000150222 001__ 150222
000150222 005__ 20180317093951.0
000150222 0247_ $$2doi$$a10.1016/j.ejcb.2010.07.007
000150222 02470 $$2ISI$$a000284197100006
000150222 037__ $$aARTICLE
000150222 245__ $$aDennexin peptides modeled after the homophilic binding sites of the neural cell adhesion molecule (NCAM) promote neuronal survival, modify cell adhesion and impair spatial learning
000150222 269__ $$a2010
000150222 260__ $$c2010
000150222 336__ $$aJournal Articles
000150222 520__ $$aNeural cell adhesion molecule (NCAM)-mediated cell adhesion results in activation of intracellular signaling cascades that lead to cellular responses such as neurite outgrowth, neuronal survival, and modulation of synaptic activity associated with cognitive processes. The crystal structure of the immunoglobulin (Ig) 1-2-3 fragment of the NCAM ectodomain has revealed novel mechanisms for NCAM homophilic adhesion. The present study addressed the biological significance of the so called dense zipper formation of NCAM. Two peptides, termed dennexinA and dennexinB, were modeled after the contact interfaces between Ig1 and Ig3 and between Ig2 and Ig2, respectively, observed in the crystal structure. Although the two dennexin peptides differed in amino acid sequence, they both modulated cell adhesion, reflected by inhibition of NCAM-mediated neurite outgrowth. Both dennexins also promoted neuronal survival, and the effect of dennexinA was independent of polysialic acid expression. Consistent with the effect of dennexinA on NCAM-mediated adhesion in vitro, the peptide impaired long-term memory retention in rats in the Morris water maze test. Thus, dennexins are novel site-specific pharmacological tools for elucidation of the role of NCAM in a variety of biological processes under normal and pathological conditions.
000150222 6531_ $$aNeural cell adhesion molecule
000150222 6531_ $$aNeurite outgrowth
000150222 6531_ $$aNeuronal survival
000150222 6531_ $$aMimetic peptides
000150222 6531_ $$aEndoN
000150222 6531_ $$aCerebellar Granule Neurons
000150222 6531_ $$aNeurite Outgrowth
000150222 6531_ $$aGrowth-Factor
000150222 6531_ $$aSubventricular Zone
000150222 6531_ $$aStructural Biology
000150222 6531_ $$aReceptor Agonist
000150222 6531_ $$aFgl-Peptide
000150222 6531_ $$aMemory
000150222 6531_ $$aIdentification
000150222 6531_ $$aApoptosis
000150222 700__ $$aKøhler, Lene B.
000150222 700__ $$aChristensen, Claus
000150222 700__ $$aRossetti, Clara
000150222 700__ $$aFantin, Martina
000150222 700__ $$0243016$$aSandi, Carmen$$g160090
000150222 700__ $$aBock, Elisabeth
000150222 700__ $$aBerezin, Vladimir
000150222 773__ $$j89$$k11$$q817-27$$tEur J Cell Biol
000150222 8564_ $$s1173530$$uhttps://infoscience.epfl.ch/record/150222/files/Kohler-Dennexin%20peptides%20modeled%20after%20the%20homo..pdf$$yn/a$$zn/a
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000150222 937__ $$aEPFL-ARTICLE-150222
000150222 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000150222 980__ $$aARTICLE