Targeted Photoswitchable Probe for Nanoscopy of Biological Structures
Dellagiacoma, Claudio; Lukinavicius, Grazvydas; Bocchio, Noelia; Banala, Sambashiva; Geissbühler, Stefan; Märki, Iwan; Johnsson, Kai; Lasser, Theo
2010
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Abstract

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged microtubule structures with ~25 nm resolution. The described probe in combination with the versatile SNAP-tag labeling opens new possibilities for imaging biological structures at the nanoscale.

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Title Targeted Photoswitchable Probe for Nanoscopy of Biological Structures
Author(s) Dellagiacoma, Claudio ; Lukinavicius, Grazvydas ; Bocchio, Noelia ; Banala, Sambashiva ; Geissbühler, Stefan ; Märki, Iwan ; Johnsson, Kai ; Lasser, Theo
Published in Chembiochem
Volume 11
Pages 1361-1363
Date 2010
ISSN 1439-4227
Keywords
Note Dellagiacoma C., Lukinavicius G. and Bocchio N. have contributed equally to this work.
DOI https://doi.org/10.1002/cbic.201000189
Other identifier(s) View record in Web of Science
Laboratories LOB
LIP
Record Appears in Scientific production and competences > SB - School of Basic Sciences > ISIC - Institute of Chemical Sciences and Engineering > LIP - Laboratory of Protein Engineering
Scientific production and competences > STI - School of Engineering > STI Archives > LOB - Laboratoire d'optique biomédicale
Peer-reviewed publications
Work produced at EPFL
Journal Articles
Published
Record creation date 2010-06-10

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