000148317 001__ 148317
000148317 005__ 20180913055739.0
000148317 022__ $$a1535-9786
000148317 02470 $$2ISI$$a000263935200013
000148317 0247_ $$2doi$$a10.1128/EC.00334-08
000148317 037__ $$aARTICLE
000148317 245__ $$aFunctional analysis of protein kinase CK2 of the human malaria parasite Plasmodium falciparum
000148317 269__ $$a2009
000148317 260__ $$bAmerican Society for Microbiology$$c2009
000148317 336__ $$aJournal Articles
000148317 520__ $$aProtein kinase CK2 (casein kinase 2) is a eukaryotic serine/threonine protein kinase with multiple substrates and roles in diverse cellular processes, including differentiation, proliferation, and translation. The mammalian holoenzyme consists of two catalytic alpha or alpha' subunits and two regulatory beta subunits. We report the identification and characterization of a Plasmodium falciparum CK2alpha orthologue, PfCK2alpha, and two PfCK2beta orthologues, PfCK2beta1 and PfCK2beta2. Recombinant PfCK2alpha possesses protein kinase activity, exhibits similar substrate and cosubstrate preferences to those of CK2alpha subunits from other organisms, and interacts with both of the PfCK2beta subunits in vitro. Gene disruption experiments show that the presence of PfCK2alpha is crucial to asexual blood stage parasites and thereby validate the enzyme as a possible drug target. PfCK2alpha is amenable to inhibitor screening, and we report differential susceptibility between the human and P. falciparum CK2alpha enzymes to a small molecule inhibitor. Taken together, our data identify PfCK2alpha as a potential target for antimalarial chemotherapeutic intervention.
000148317 6531_ $$aRegulatory Subunit
000148317 6531_ $$aCrystal-Structure
000148317 6531_ $$aCk2-Beta Subunit
000148317 6531_ $$aBeta-Subunit
000148317 6531_ $$aLife-Cycle
000148317 6531_ $$aIi-Alpha
000148317 6531_ $$aPhosphorylation
000148317 6531_ $$aSite
000148317 6531_ $$aInhibitors
000148317 6531_ $$aSubstrate
000148317 700__ $$aHolland, Zoë
000148317 700__ $$aPrudent, Renaud
000148317 700__ $$aReiser, Jean-Baptiste
000148317 700__ $$aCochet, Claude
000148317 700__ $$0243705$$aDoerig, Christian$$g191299
000148317 773__ $$j8$$k3$$q388-97$$tEukaryotic cell
000148317 8564_ $$s1374537$$uhttps://infoscience.epfl.ch/record/148317/files/Holland-Z_2009.pdf$$yPublisher's version$$zn/a
000148317 909C0 $$0252274$$pINSERM-EPFL$$xU12184
000148317 909CO $$ooai:infoscience.tind.io:148317$$particle
000148317 917Z8 $$x182396
000148317 937__ $$aEPFL-ARTICLE-148317
000148317 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000148317 980__ $$aARTICLE