000147793 001__ 147793
000147793 005__ 20180913055720.0
000147793 022__ $$a0363-6135
000147793 037__ $$aARTICLE
000147793 245__ $$aRole of smooth muscle cells on endothelial cell cytosolic free calcium in porcine coronary arteries
000147793 269__ $$a2001
000147793 260__ $$c2001
000147793 336__ $$aJournal Articles
000147793 520__ $$aWe tested the hypothesis that the cytosolic free calcium concentration in endothelial cells is under the influence of the smooth muscle cells in the coronary circulation. In the left descending branch of porcine coronary arteries, cytosolic free calcium concentration ([Ca(2+)](i)) was estimated by determining the fluorescence ratio of two calcium probes, fluo 4 and fura red, in smooth muscle and endothelial cells using confocal microscopy. Acetylcholine and potassium, which act directly on smooth muscle cells to increase [Ca(2+)](i), were found to indirectly elevate [Ca(2+)](i) in endothelial cells; in primary cultures of endothelial cells, neither stimulus affected [Ca(2+)](i), yet substance P increased the fluorescence ratio twofold. In response to acetylcholine and potassium, isometric tension developed by arterial strips with intact endothelium was attenuated by up to 22% (P < 0.05) compared with strips without endothelium. These findings suggest that stimuli that increase smooth muscle [Ca(2+)](i) can indirectly influence endothelial cell function in porcine coronary arteries. Such a pathway for negative feedback can moderate vasoconstriction and diminish the potential for vasospasm in the coronary circulation.
000147793 700__ $$aBudel, S.
000147793 700__ $$aSchuster, A.
000147793 700__ $$aStergiopoulos, N.
000147793 700__ $$0240339$$aMeister, J. J.$$g105816
000147793 700__ $$0243846$$aBény, J. L.$$g148872
000147793 773__ $$j281$$k3$$qH1156-62$$tAmerican journal of physiology. Heart and circulatory physiology
000147793 909C0 $$0252255$$pLCB$$xU10146
000147793 909CO $$ooai:infoscience.tind.io:147793$$pSB$$particle
000147793 937__ $$aEPFL-ARTICLE-147793
000147793 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000147793 980__ $$aARTICLE