000147737 001__ 147737
000147737 005__ 20181203021843.0
000147737 0247_ $$2doi$$a10.1529/biophysj.108.131136
000147737 022__ $$a00063495
000147737 022__ $$a15420086
000147737 02470 $$2ISI$$a000258826900013
000147737 037__ $$aARTICLE
000147737 245__ $$aCalcium dynamics and vasomotion in arteries subject to isometric, isobaric, and isotonic conditions
000147737 260__ $$c2008
000147737 269__ $$a2008
000147737 336__ $$aJournal Articles
000147737 520__ $$aIn vitro, different techniques are used to study the smooth muscle cells' calcium dynamics and contraction/relaxation mechanisms on arteries. Most experimental studies use either an isometric or an isobaric setup. However, in vivo, a blood vessel is neither isobaric nor isometric nor isotonic, as it is continuously submitted to intraluminal pressure variations arising from heart beat. We use a theoretical model of the smooth muscle calcium and arterial radius dynamics to determine whether results may be considerably different depending on the experimental conditions (isometric, isobaric, isotonic, or cyclic pressure variations). We show that isobaric conditions appear to be more realistic than isometric or isotonic situations, as the calcium dynamics is similar under cyclic intraluminal pressure variations (in vivo-like situation) and under a constant pressure (isobaric situation). The arterial contraction is less pronounced in isotonic than in isobaric conditions, and the vasoconstrictor sensitivity higher in isometric than isobaric or isotonic conditions, in agreement with experimental observations. Interestingly, the model predicts that isometric conditions may generate artifacts like the coexistence of multiple stable states. We have verified this model prediction experimentally using rat mesenteric arteries mounted on a wire myograph and stimulated with phenylephrine. © 2008 by the Biophysical Society.
000147737 700__ $$aKoenigsberger, M.
000147737 700__ $$aSauser, R.
000147737 700__ $$0243849$$g128638$$aSeppey, D.
000147737 700__ $$0243846$$g148872$$aBény, J.-L.
000147737 700__ $$aMeister, J.-J.$$g105816$$0240339
000147737 773__ $$j95$$tBiophysical Journal$$k6$$q2728-2738
000147737 909C0 $$xU10146$$0252255$$pLCB
000147737 909CO $$pSB$$particle$$ooai:infoscience.tind.io:147737
000147737 937__ $$aEPFL-ARTICLE-147737
000147737 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000147737 980__ $$aARTICLE