000147726 001__ 147726
000147726 005__ 20181203021843.0
000147726 0247_ $$2doi$$a10.1016/j.bpj.2009.07.020
000147726 022__ $$a00063495
000147726 022__ $$a15420086
000147726 02470 $$2ISI$$a000270586000006
000147726 037__ $$aARTICLE
000147726 245__ $$aActin-myosin viscoelastic flow in the keratocyte lamellipod
000147726 260__ $$c2009
000147726 269__ $$a2009
000147726 336__ $$aJournal Articles
000147726 520__ $$aThe lamellipod, the locomotory region of migratory cells, is shaped by the balance of protrusion and contraction. The latter is the result of myosin-generated centripetal flow of the viscoelastic actin network. Recently, quantitative flow data was obtained, yet there is no detailed theory explaining the flow in a realistic geometry. We introduce models of viscoelastic actin mechanics and myosin transport and solve the model equations numerically for the flat, fan-shaped lamellipodial domain of keratocytes. The solutions demonstrate that in the rapidly crawling cell, myosin concentrates at the rear boundary and pulls the actin network inward, so the centripetal actin flow is very slow at the front, and faster at the rear and at the sides. The computed flow and respective traction forces compare well with the experimental data. We also calculate the graded protrusion at the cell boundary necessary to maintain the cell shape and make a number of other testable predictions. We discuss model implications for the cell shape, speed, and bi-stability. © 2009 by the Biophysical Society.
000147726 6531_ $$aRetrograde Flow
000147726 6531_ $$aCell-Migration
000147726 6531_ $$aFish Keratocytes
000147726 6531_ $$aContinuum Model
000147726 6531_ $$aLeading-Edge
000147726 6531_ $$aMotility
000147726 6531_ $$aDynamics
000147726 6531_ $$aShape
000147726 6531_ $$aPolarization
000147726 6531_ $$aAdhesions
000147726 700__ $$aRubinstein, B.
000147726 700__ $$0243650$$aFournier, M. F.$$g176552
000147726 700__ $$aJacobson, K.
000147726 700__ $$0243647$$aVerkhovsky, A. B.$$g129621
000147726 700__ $$aMogilner, A.
000147726 773__ $$j97$$k7$$q1853-1863$$tBiophysical Journal
000147726 909C0 $$0252255$$pLCB$$xU10146
000147726 909CO $$ooai:infoscience.tind.io:147726$$pSB$$particle
000147726 937__ $$aEPFL-ARTICLE-147726
000147726 973__ $$aEPFL$$rNON-REVIEWED$$sPUBLISHED
000147726 980__ $$aARTICLE