Acoustophoretic Synchronization of Mammalian Cells in Microchannels

We report the first use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. The acoustophoretic cell synchronization (ACS) device utilizes volume-dependent acoustic radiation force within a microchannel to selectively purify target cells of desired phase from an asynchronous mixture based on cell cycle dependent fluctuations in size. We show that ultrasonic separation allows for gentle, scalable, and label-free synchronization with high G1 phase synchrony (∼84%) and throughput (3 × 106 cells/h per microchannel).


Published in:
Analytical Chemistry, 82, 7, 3094-3098
Year:
2010
Publisher:
American Chemical Society
ISSN:
1520-6882
Keywords:
Laboratories:


Note: The status of this file is: EPFL only


 Record created 2010-03-23, last modified 2018-03-17

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