000147377 001__ 147377
000147377 005__ 20190316234741.0
000147377 0247_ $$2doi$$a10.1021/la800438q
000147377 022__ $$a1520-5827
000147377 02470 $$2ISI$$a000256656500031
000147377 037__ $$aARTICLE
000147377 245__ $$aTemperature-Dependent Elasticity of Microtubules
000147377 269__ $$a2008
000147377 260__ $$bAmerican Chemical Society$$c2008
000147377 336__ $$aJournal Articles
000147377 520__ $$aCentral to the biological function of microtubules. is their ability to modify their length which occurs by addition and removal of subunits at the ends of the polymer, both in vivo and in vitro. This dynamic behavior is strongly influenced by temperature. Here, we show that the lateral interaction between tubulin subunits forming microtubule is strongly temperature dependent. Microtubules deposited on prefabricated substrates were deformed in an atomic force microscope during imaging, in two different experimental geometries. Microtubules were modeled as anisotropic, with the Young's modulus corresponding to the resistance of protofilaments to stretching and the shear modulus describing the weak interaction between the protofilaments. Measurements involving radial compression of raicrotubules deposited on flat mica confirm that microtubule elasticity depends on the temperature. Bending measurements performed on microtubules deposited on lithographically fabricated substrates show that this temperature dependence is due to changing shear modulus, implying that the lateral interaction between the protofilaments is strongly determined by the temperature. These measurements are in good agreement with previously repot led measurements of the disassembly rate of microtubules, demonstrating that the mechanical and dynamic properties of microtubules are closely related.
000147377 6531_ $$amicrotubules
000147377 6531_ $$ananomechanics
000147377 6531_ $$aatomic force microscopy
000147377 700__ $$0240306$$g133331$$aKis, A.
000147377 700__ $$0240307$$g150334$$aKasas, S.
000147377 700__ $$0240620$$g105590$$aKulik, A. J.
000147377 700__ $$aCatsicas, S.$$g134380$$0240092
000147377 700__ $$aForró, L.$$g105148$$0240227
000147377 773__ $$j24$$tLangmuir$$k12$$q6176-6181
000147377 8564_ $$uhttps://infoscience.epfl.ch/record/147377/files/la800438q%252Elowlink%252Epdf_v03.pdf$$s248033$$yPublisher's version
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000147377 909C0 $$0252082$$pLNNME
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000147377 917Z8 $$x102085
000147377 937__ $$aEPFL-ARTICLE-147377
000147377 937__ $$aLNNME-ARTICLE-2008-042
000147377 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000147377 980__ $$aARTICLE