Interaction of mitochondrial creatine kinase with model membranes: A monolayer study

The interaction of mitochondrial creatine kinase (Mi-CK; EC 2.7.3.2) with phospholipid monolayers and spread mitochondrial membranes at the air/water interface has been investigated. It appeared that Mi-CK penetrated into these monolayers as evidenced by an increase in surface pressure upon incorporation of Mi-CK. The increase in surface pressure was dependent on (1) the amount and (2) the oligomeric form of Mi-CK in the subphase, as well as on (3) the initial surface pressure and (4) the phospholipid composition of the monolayer. In this experimental system Mi-CK was able to interact equally well with both inner and outer mitochondrial membranes.


Published in:
FEBS letters, 281, 1-2, 123-9
Year:
1991
Publisher:
Elsevier
ISSN:
0014-5793
Keywords:
Laboratories:




 Record created 2010-03-16, last modified 2018-09-13


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