Super-resolved position and orientation estimation of fluorescent dipoles using 3D steerable filters

Fluorescence localization microscopy techniques such as PALM and STORM achieve super-resolution by sequentially imaging sparse subsets of fluorophores, which are localized by means of Gaussian-based localization. Applied to fixed fluorophores, with dipole radiation characteristics, this can lead to an estimation bias in the range of 5-20nm. We introduce a method for the joint estimation of position and orientation of single fluorophores, based on an accurate image formation model expressed as a 3-D steerable filter. In addition to avoiding bias in PALM/STORM, it can remove the ambiguity of the orientation factor for studying dipole-dipole interactions at the single-molecule level.


Presented at:
BIOS 2010 - Single Molecule Spectroscopy and Imaging III, San Francisco, January 23-28 2010
Year:
2010
Publisher:
Spie-Int Soc Optical Engineering, Po Box 10, Bellingham, Wa 98227-0010 Usa
Keywords:
Note:
Invited Talk
Laboratories:




 Record created 2010-02-17, last modified 2018-09-13


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