Single-molecule avidin-biotin association reaction studied by force-clamp spectroscopy

Using single-molecule force-clamp spectroscopy, where the distance between the AFM tip and the sample surface is fixed and a few parallel avidin-biotin complexes are kept stretched by a certain force, we were able to observe the formation of single avidin-biotin bonds. Perspectives to use such all approach to study association reactions at single-molecule level in the conditions resembling those characteristic for some processes in vivo (e.g. virus-cell membrane attachment) are briefly discussed. (C) 2008 Elsevier B.V. All rights reserved.


Published in:
ULTRAMICROSCOPY, 108, 1135-1139
Presented at:
9th International Conference on Scanning Probe Microscopy, Sensors and Nanostructures, Jeju, SOUTH KOREA, JUN 10-14, 2007
Year:
2008
Keywords:
Laboratories:




 Record created 2010-02-09, last modified 2018-09-13


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)