000144041 001__ 144041
000144041 005__ 20190416055737.0
000144041 037__ $$aPOST_TALK
000144041 245__ $$aDesign and Optimization of a Lactate Amperometric Biosensor based on Lactate Oxidase and Multi Walled-Carbon Nanotubes
000144041 269__ $$a2008
000144041 260__ $$c2008
000144041 336__ $$aPosters
000144041 520__ $$aLactate concentration in physiological fluids is an indicator of the energy production under anaerobic conditions of the whole organism [1]. Normal value for lactate level in blood ranges from 0.5 – 2.5 mM. Blood lactate values exceeding 7-8 mM are sometimes associated with a fatal outcome. The lactate level in blood is elevated under number of conditions. It is a major indicator of ischemic conditions, e.g. under the category of tissue hypoxia, the individual causes include shock (hypovolemic, cardiogenic or endotoxic), respiratory failure (asphyxia) and severe congestive heart failure. Gauging blood lactate is also relevant for the results of exercise and athletic performance [2]. Several approaches were made to develop a number of improved methods for lactic acid determination, such ad optic techniques (HPLC, mass spectroscopy, ion exchange chromatography, etc.) and electrochemical techniques (potentiometric and voltammetric detections, FET-based sensors, etc.) [1]. The present study is focused on development of technologies for lactate detection based on amperometric measures employing lactate oxidase (LOD). The project’s aim is double: the development of biosensors which can be integrated in Petri dishes for monitoring cell cultures, on one hand, and which can be integrated in point-of-care devices for human monitoring in personalized therapies, on the other hand. In both the cases, analysis of more than one substrate at the same time will be considered. Nanotechnology may be used in the optimization of electrodes with carbon nanotubes (CNT). Previous studies showed that CNTs can highly improve the electrocatalytic activity in electrochemical devices for monitoring metabolites [3].
000144041 700__ $$aBoero, C
000144041 700__ $$0242413$$g182237$$aCarrara, S
000144041 700__ $$aDe Micheli, G$$g167918$$0240269
000144041 7112_ $$dNovember 17-19, 2008$$cMontreux$$aNanoTech 2008
000144041 8564_ $$uhttps://infoscience.epfl.ch/record/144041/files/Abstarct-Montreux%20Nanotech%202008.pdf$$zn/a$$s169063
000144041 909C0 $$xU11140$$0252283$$pLSI1
000144041 909CO $$pposter$$ooai:infoscience.tind.io:144041$$qGLOBAL_SET$$pSTI$$pIC
000144041 937__ $$aEPFL-POSTER-144041
000144041 973__ $$sPUBLISHED$$aEPFL
000144041 980__ $$aPOSTER