000143382 001__ 143382
000143382 005__ 20181228151104.0
000143382 0247_ $$2doi$$a10.1016/0197-0186(80)90071-6
000143382 037__ $$aARTICLE
000143382 245__ $$aThe isolated retina of mammals: A useful preparation for enzymatic-(adenylyl cyclase) and/or binding studies of dopamine receptors
000143382 269__ $$a1980
000143382 260__ $$c1980
000143382 336__ $$aJournal Articles
000143382 520__ $$aRabbit and bovine retinae were used for investigations in vitro related to dopamine receptors. Dose-dependent stimulations of cyclic AMP formation induced by dopamine (or dopamine-like drugs including ADTN and some ergot alkaloid derivatives), which can be blocked by dopamine antagonists (neuroleptics), lithium or d-LSD, were found either in intact retinae or in homogenates. The neuroleptic sulpiride was the only exception since it was devoid of inhibitory effects. Binding studies of retinal dopamine receptors were also achieved with homogenates. By using [(3)H]spiroperidol as a ligand, saturability (in the amolar range), stereospecificity (by using butaclamol- or thioxanthene-isomers), tissue linearity, and pH- or temperature-dependence were all demonstrated. Furthermore, displacement curves of [(3)H]spiroperidol stereospecifically bound generated by various dopamineand/or catecholamine-related drugs seem to indicate that dopamine receptors are the only monoamine receptors in this tissue and that a coupling possibly exists between binding- and catalytic-sites. These results suggest that by studying both dopamine-sensitive adenylyl cyclase and drug-induced displacement of [(3)H]spiroperidol binding the mammalian retina is appropriate to selectively characterize the type of action of various drugs with CNS dopamine receptors
000143382 6531_ $$aRetina dopamine receptors
000143382 6531_ $$aadenylyl cyclase
000143382 6531_ $$abinding studies
000143382 6531_ $$aneuroleptics
000143382 6531_ $$aergot alkaloids
000143382 700__ $$aSchorderet, M
000143382 700__ $$g134990$$aMagistretti, P J$$0243698
000143382 773__ $$j1$$tNeurochemistry International$$q337-353
000143382 909C0 $$xU11150$$0252265$$pLNDC
000143382 909CO $$qSV$$particle$$ooai:infoscience.tind.io:143382
000143382 937__ $$aLNDC-ARTICLE-2010-003
000143382 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000143382 980__ $$aARTICLE