000143027 001__ 143027
000143027 005__ 20190604162132.0
000143027 0247_ $$2doi$$a10.1371/journal.pone.0002588
000143027 037__ $$aARTICLE
000143027 245__ $$aIntestinal bacteria condition dendritic cells to promote IgA production
000143027 269__ $$a2008
000143027 260__ $$c2008
000143027 336__ $$aJournal Articles
000143027 500__ $$aMassacand, Joanna C Kaiser, Patrick Ernst, Bettina Tardivel, Aubry Burki, Kurt Schneider, Pascal Harris, Nicola L Research Support, Non-U.S. Gov't United States PloS one PLoS One. 2008 Jul 2;3(7):e2588.
000143027 520__ $$aImmunoglobulin (Ig) A represents the predominant antibody isotype produced at the intestinal mucosa, where it plays an important role in limiting the penetration of commensal intestinal bacteria and opportunistic pathogens. We show in mice that Peyer's Patch-derived dendritic cells (PP-DC) exhibit a specialized phenotype allowing the promotion of IgA production by B2 cells. This phenotype included increased expression of the retinaldehyde dehydrogenase 1 (RALDH1), inducible nitric oxide synthase (iNOS), B cell activating factor of the tumor necrosis family (BAFF), a proliferation-inducing ligand (APRIL), and receptors for the neuropeptide vasoactive intestinal peptide (VIP). The ability of PP-DC to promote anti-CD40 dependent IgA was partially dependent on retinoic acid (RA) and transforming growth factor (TGF)-beta, whilst BAFF and APRIL signaling were not required. Signals delivered by BAFF and APRIL were crucial for CD40 independent IgA production, although the contribution of B2 cells to this pathway was minimal. The unique ability of PP-DC to instruct naive B cells to differentiate into IgA producing plasma cells was mainly imparted by the presence of intestinal commensal bacteria, and could be mimicked by the addition of LPS to the culture. These data indicate that exposure to pathogen-associated molecular patterns present on intestinal commensal bacteria condition DC to express a unique molecular footprint that in turn allows them to promote IgA production.
000143027 6531_ $$aAnimals
000143027 6531_ $$aCell Differentiation
000143027 6531_ $$aDendritic Cells/*immunology
000143027 6531_ $$aFlow Cytometry
000143027 6531_ $$aImmunoglobulin A/*biosynthesis/immunology
000143027 6531_ $$aIntestine, Small/*microbiology
000143027 6531_ $$aMice
000143027 6531_ $$aMice, Inbred C57BL
000143027 6531_ $$aPeyer's Patches/immunology/microbiology
000143027 6531_ $$aPhenotype
000143027 700__ $$0243701$$g196766$$aMassacand, J. C.
000143027 700__ $$aKaiser, P.
000143027 700__ $$aErnst, B.
000143027 700__ $$aTardivel, A.
000143027 700__ $$aBurki, K.
000143027 700__ $$aSchneider, P.
000143027 700__ $$0243703$$g196676$$aHarris, N. L.
000143027 773__ $$j3$$tPLoS One$$k7$$qe2588
000143027 8564_ $$uhttps://infoscience.epfl.ch/record/143027/files/2008_Intestinal%20bacteria%20condition%20dendritic%20cells%20to%20promote%20IgA%20production.pdf$$zn/a$$s413474
000143027 909C0 $$xU12123$$0252266$$pUPHARRIS
000143027 909CO $$ooai:infoscience.tind.io:143027$$qSV$$qGLOBAL_SET$$particle
000143027 937__ $$aUPHARRIS-ARTICLE-2010-029
000143027 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000143027 980__ $$aARTICLE