Deliberate removal of T cell help improves virus-neutralizing antibody production
The B cell response to lymphocytic choriomeningitis virus is characterized by a CD4(+) T cell-dependent polyclonal hypergammaglobulinemia and delayed formation of virus-specific neutralizing antibodies. Here we provide evidence that, paradoxically, because of polyclonal B cell activation, virus-specific T cell help impairs the induction of neutralizing antibody responses. Experimental reduction in CD4(+) T cell help in vivo resulted in potent neutralizing antibody responses without impairment of CD8(+) T cell activity. These unexpected consequences of polyclonal B cell activation may affect vaccine strategies and the treatment of clinically relevant chronic bacterial, parasitic and viral infections in which hypergammaglobulinemia is regularly found.
Keywords: Animals ; Antibody Formation/*immunology ; Arenaviridae Infections/immunology ; B-Lymphocytes/*immunology ; CD4-Positive T-Lymphocytes/*immunology ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Lymphocyte Activation/*immunology ; Lymphocytic choriomeningitis virus/immunology ; Mice
Recher, Mike Lang, Karl S Hunziker, Lukas Freigang, Stefan Eschli, Bruno Harris, Nicola L Navarini, Alexander Senn, Beatrice M Fink, Katja Lotscher, Marius Hangartner, Lars Zellweger, Raphael Hersberger, Martin Theocharides, Alexandre Hengartner, Hans Zinkernagel, Rolf M Research Support, Non-U.S. Gov't United States Nature immunology Nat Immunol. 2004 Sep;5(9):934-42. Epub 2004 Aug 8.
Record created on 2010-01-06, modified on 2016-08-08