000142360 001__ 142360
000142360 005__ 20190812205347.0
000142360 0247_ $$2doi$$a10.1016/j.mod.2009.06.432
000142360 037__ $$aCONF
000142360 245__ $$aSomitogenesis and development of primary motor neurons: The role of the homeobox uncx4.1
000142360 269__ $$a2009
000142360 260__ $$bElsevier Science BV$$c2009
000142360 336__ $$aConference Papers
000142360 500__ $$aMeeting Abstract
000142360 520__ $$aThe gene uncx4.1 is a paired-type homeobox transcription factor, expressed during zebrafish embryonic development in branchial arches, somites, CNS and pronephric ducts. In particular, the expression at the somite level is detectable from 5ss stage and becomes progressively posteriorly and ventrally restricted to the presumptive myoblast cells in later stages. Interestingly, the progressive restriction of uncx4.1 activity anticipates and accompanies the appearance of the first outgrowing primary motor axons. Indeed, after uncx4.1 expression is not detected anymore in medial somitic cells, a ventrally projecting CaP motor axons emerge from the ventro-lateral spinal cord. In relation with muscle differentiation, double hybridizations showed that uncx4.1 expression precedes myoD activation in muscle precursor cells. Moreover, co-localization of uncx4.1 mRNA and Myosin protein (fibroblast marker) reveals a complementary distribution of the two molecules, suggesting that uncx4.1 activity steps back from maturing muscle cells. uncx4.1 seems to be negatively regulated by shh pathway, indeed shh overexpression abolishes uncx4.1 transcription. Analyses of the shh pathway zebrafish mutants (floating head (flh), cyclops (nodal), sonic you (shh) and you-too (gli2)) showed that the expression of the uncx4.1 gene extends abnormally from dorsal to ventral within somitic compartments, confirming the negative regulation. Currently, uncx4.1 functional characterization is performed by a morpholino mediated strategy. Spatio-temporal dynamics of uncx4.1 expression, along with functional experimental evidences, suggest a double role for the uncx4.1 gene, in guiding outgrowth of motor axons, and regulating cell cycle in muscle progenitors at the margin of each somite.
000142360 700__ $$aFortunato, A. E.
000142360 700__ $$aKondoh, T.
000142360 700__ $$0240276$$g141236$$aDuboule, D.
000142360 700__ $$aHolder, N.
000142360 700__ $$aWilson, S.
000142360 700__ $$aSordino, P.
000142360 7112_ $$dSeptember 6-10 2009$$cEdinburg, Scotland$$a16th Annual Conference of the International-Society-of-Development-Biologists
000142360 773__ $$tMechanisms of Development$$qS180-S181
000142360 909C0 $$xU11705$$pUPDUB$$0252099
000142360 909CO $$pconf$$pSV$$ooai:infoscience.tind.io:142360
000142360 937__ $$aUPDUB-CONF-2009-003
000142360 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000142360 980__ $$aCONF