Low back pain is one of the most prevailing medical concerns and can often be associated with the degeneration of the intervertebral disc. This degeneration results in a loss of biological and mechanical properties, especially a loss of viscoelastic properties. In the present study a correlation between viscoelasticity of a hydrogel-cell construct and the extracellular matrix production is researched. Therefore, a model that mimics the real intervertebral disc, composed of a confined hydrogel, is developed. The first part of the project is focalized on the biocompatibility of the confinement chamber. The second part will focalize on the extracellular matrix production from a hydrogel-cell construct submitted to dynamical mechanical stimulations and the viscoelastic properties of theses constructs. Thereby, a multiwell stimulator was developed and 4 different stimulations were performed during 1 month. The stimulations do not seem having great effects on the extracellular matrix production. This study demonstrates also the feasibility to stimulate hydrogel-cell constructs for 1 month, and to use a novel device for stimulation: the multiwell stimulator.