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000142245 037__ $$aCONF
000142245 245__ $$aSeparation of antibiotic-treated mycobacterial subpopulations using multiple-frequency dielectrophoresis
000142245 269__ $$a2009
000142245 260__ $$c2009
000142245 336__ $$aConference Papers
000142245 520__ $$aExposure of a bacterial culture to an antibiotic or other lethal stress typically results in the generation of sub-populations of live cells ("persisters") and dead cells, and, in some cases, viable but non-culturable cells. Studies aimed at understanding the mechanisms of antibiotic killing and bacterial persistence will require the purification and analysis of each of these sub-populations in isolation. This paper reports a method based on continuous-flow dielectrophoresis to separate live and dead cells from antibiotic-treated bacterial cultures. Our experiments were carried out on Mycobacterium smegmatis, a model organism that is closely related to the etiological agent of human tuberculosis.
000142245 6531_ $$aDielectrophoresis
000142245 6531_ $$apersisters
000142245 6531_ $$aM.smegmatis
000142245 6531_ $$acontinuous flow speration
000142245 700__ $$aElitas, M.
000142245 700__ $$0242543$$g178347$$aValero, Ana
000142245 700__ $$0240218$$g157067$$aBraschler, Thomas
000142245 700__ $$aDhar, N.
000142245 700__ $$aMcKinney, J. D.
000142245 700__ $$aRenaud, Philippe$$0240219$$g107144
000142245 7112_ $$dNov. 1-5, 2009$$cJeju, Korea$$a13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS)
000142245 8564_ $$uhttps://infoscience.epfl.ch/record/142245/files/188-M60F.pdf$$zn/a$$s211038
000142245 909C0 $$xU10324$$0252064$$pLMIS4
000142245 909CO $$ooai:infoscience.tind.io:142245$$qGLOBAL_SET$$pconf$$pSTI
000142245 937__ $$aLMIS4-CONF-2009-009
000142245 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000142245 980__ $$aCONF