Surface-Functionalized Ultrasmall Superparamagnetic Nanoparticles as Magnetic Delivery Vectors for Camptothecin
The linking of therapeutic drugs to ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) allowing intracellular release of the active drug via cell-specific mechanisms would achieve tumor-selective magnetically-enhanced drug delivery. To validate this concept, we covalently attached the anticancer drug camptothecin (CPT) to biocompatible USPIOs (iron oxide core, 9–10 nm ; hydrodynamic diameter, 52 nm) coated with polyvinylalcohol/polyvinylamine (PVA/aminoPVA). A bifunctional, end-differentiated dicarboxylic acid linker allowed the attachment of CPT to the aminoPVA as a biologically labile estersubstrate for cellular esterases at one end, and as an amide at the other end. These CPT–USPIO conjugates exhibited antiproliferative activity in vitro against human melanoma cells. The intracellular localization of CPT–USPIOs was confirmed by transmission electron microscopy (iron oxide core), suggesting localization in lipid vesicles, and by fluorescence microscopy (CPT). An external static magnetic field applied during exposure increased melanoma cell uptake of the CPT–USPIOs.
Keywords: Ultrasmall ; antitumor agents ; chemical functionalization ; drug delivery ; nanoparticles ; Superparamagnetic ; Magnetic ; Vectors ; Camptothecin ; Iron-Oxide Nanoparticles ; Locoregional Cancer-Treatment ; Drug-Delivery ; Biomedical Applications ; Chemotherapeutic Drugs ; Polymeric Micelles ; In-Vitro ; Therapy ; Cells ; Systems
Record created on 2009-08-13, modified on 2016-08-08