Digital holographic microscopy for study cellular dynamics

Based on an original numerical reconstruction algorithm (E. Cuche et al. Appl. Opt. 38, 6994 1999), we have developed a Digital Holographic Microscope (DHM), in a transmission mode, allowing to investigate noninvasively cellular structures and dynamics. DHM images of living cells in culture are presented. They represent the distribution of the optical path length over the cell, which contains information concerning both the cellular morphometry and the intracellular refractive index, and which has been measured with a sub-wavelength accuracy.


Published in:
Novel Optical Instrumentation for Biomedical Applications II
Presented at:
European Conferences on Biomedical Optics, Munich, June 17, 2005
Year:
2005
Publisher:
SPIE
Keywords:
Laboratories:




 Record created 2009-08-03, last modified 2018-03-17

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