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research article
Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection
2009
We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485–670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6–1 μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.
Type
research article
Web of Science ID
WOS:000264522400035
Authors
Kühn, Jonas
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Moratal, Corinne
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Marquet, Pierre
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Publication date
2009
Published in
Volume
34
Issue
5
Start page
653
End page
655
Peer reviewed
REVIEWED
EPFL units
Available on Infoscience
July 20, 2009
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