Submicrometer tomography of cells by multiple-wavelength digital holographic microscopy in reflection

We present first results on a method enabling mechanical scanning-free tomography with submicrometer axial resolution by multiple-wavelength digital holographic microscopy. By sequentially acquiring reflection holograms and summing 20 wavefronts equally spaced in spatial frequency in the 485–670 nm range, we are able to achieve a slice-by-slice tomographic reconstruction with a 0.6–1 μm axial resolution in a biological medium. The method is applied to erythrocytes investigation to retrieve the cellular membrane profile in three dimensions.


Published in:
Opt. Lett., 34, 5, 653-655
Year:
2009
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 Record created 2009-07-20, last modified 2018-01-28

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