Analysis of cellular structure and dynamics with digital holographic microscopy

Digital holographic microscopy (DHM) is a technique that allows obtaining, from a single recorded hologram, quantitative phase image of living cell with interferometric accuracy. Specifically, the optical phase shift induced by the specimen on the transmitted wave front can be regarded as a powerful endogenous contrast agent, depending on both the thickness and the refractive index of the sample. We discuss some approaches allowing to directly obtain separate measurements of the thickness and the refractive index (RI) of a given living cell from the phase signal. © 2007 SPIE-OSA.


Published in:
Biophotonics 2007: Optics in Life Science, 6633
Presented at:
European Conferences on Biomedical Optics, Munich, June 17-21, 2007
Year:
2007
Publisher:
SPIE
ISSN:
1605-7422
ISBN:
9780819467775
Keywords:
Laboratories:




 Record created 2009-07-20, last modified 2018-09-13

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