Cell refractive index tomography by digital holographic microscopy

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the threedimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.


Published in:
Optics Letters, 31, 2, 178-180
Year:
2006
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 Record created 2009-07-20, last modified 2018-12-03

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