000139719 001__ 139719
000139719 005__ 20181203021649.0
000139719 0247_ $$2doi$$a10.1364/OPEX.13.009361
000139719 02470 $$2ISI$$a000233334900026
000139719 037__ $$aARTICLE
000139719 245__ $$aMeasurement of the integral refractive index and dynamic cell morphometry  of living cells with digital holographic microscopy
000139719 269__ $$a2005
000139719 260__ $$c2005
000139719 336__ $$aJournal Articles
000139719 520__ $$aWe have developed a digital holographic microscope (DHM), in a transmission  mode, adapted to the quantitative study of cellular dynamics. Living cells in  culture are optically probed by measuring the phase shift they produce on the  transmitted wave front. The high temporal stability of the phase signal,  equivalent to ?/1800, and the low acquisition time (~20µs) enable to monitor  cellular dynamics processes. An experimental procedure allowing to calculate  both the integral refractive index and the cellular thickness (morphometry)  from the measured phase shift is presented. Specifically, the method has  been applied to study the dynamics of neurons in culture during a hypotonic  stress. Such stress produces a paradoxical decrease of the phase which can  be entirely resolved by applying the methodological approach described in  this article; indeed the method allows to determine independently the  thickness and the integral refractive index of cells.
000139719 6531_ $$a[MVD]
000139719 6531_ $$aHolography : Computer holography
000139719 6531_ $$aInstrumentation, measurement, and metrology : Metrology
000139719 6531_ $$aInstrumentation, measurement, and metrology : Phase measurement
000139719 6531_ $$aMedical optics and biotechnology : Cell analysis
000139719 700__ $$0240010$$aRappaz, Benjamin$$g168594
000139719 700__ $$aMarquet, Pierre
000139719 700__ $$0240772$$aCuche, Etienne$$g104844
000139719 700__ $$aEmery, Yves
000139719 700__ $$0240009$$aDepeursinge, Christian$$g104931
000139719 700__ $$aMagistretti, Pierre J.
000139719 773__ $$j13$$k23$$q9361 - 9373$$tOptics Express
000139719 8564_ $$uhttp://www.opticsinfobase.org/oe/abstract.cfm?URI=OPEX-13-23-9361$$zURL
000139719 909C0 $$0252008$$pLOA$$xU10346
000139719 909CO $$ooai:infoscience.tind.io:139719$$pSTI$$particle
000139719 937__ $$aLOA-ARTICLE-2005-008
000139719 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000139719 980__ $$aARTICLE