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research article

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Nallet, S
•
Amacker, M
•
Westerfeld, N
Show more
2009
Vaccine

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV fusion protein (rRSV-F) are described. The recombinant protein was produced by transient gene expression (TGE) in suspension-adapted human embryonic kidney cells (HEK-293E) in 4L orbitally shaken bioreactors. It was then purified and formulated in immunostimulating reconstituted influenza virosomes (IRIVs). The candidate vaccine induced anti-RSV-F neutralizing antibodies in mice, and challenge studies in cotton rats are ongoing. If successful in preclinical and clinical trials, this will be the first recombinant subunit vaccine produced by large-scale TGE in mammalian cells.

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Type
research article
DOI
10.1016/j.vaccine.2009.06.019
Web of Science ID

WOS:000271922700006

Author(s)
Nallet, S
Amacker, M
Westerfeld, N
Baldi, L  
König, I
Hacker, DL  
Zaborosch, C
Zurbriggen, R
Wurm, FM  
Date Issued

2009

Published in
Vaccine
Volume

27

Issue

46

Start page

6415

End page

6419

Subjects

Transient transfection

•

Recombinant protein

•

RSV vaccine

•

Virosomes

•

Large-Scale Production

•

Influenza Virosomes

•

Delivery-System

•

Efficient

•

Immunity

•

Purification

•

Technology

•

Infection

•

Children

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBTC  
Available on Infoscience
July 15, 2009
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/41349
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