In this paper, we report on the first use of an amperometric fluidic microchip array for the examination of nitric oxide in solution. The array chip is composed of 36 working platinum electrodes on a glass substrate. The electrodes have a diameter of 50 μm and are separated by 500 μm. The array chip is integrated within a flowing cell to obtain a fluidic-type sensing device. Two preliminary tests were performed. The first one consisted in assessing the fluidic set-up by using potassium ferrocyanide as test analyte. The second test was aimed at achieving the modification of the surface of the working electrodes by electrodepositing nickel tetrasulfonated phthalocyanine and Nafion® layers to show that the fluidic sensing device can be adapted to the analysis of nitric oxide in solution. © 2005 Elsevier B.V. All rights reserved.