Enzyme modified microband electrodes: cross-talk effects and their elimination

One microband of an array of four microband electrodes (1 mm long and 25 μm wide with a 25 μm gap) was modified with glucose oxidase by direct electrochemically assisted immobilisation, giving a stable microbiosensor with an apparent Michaelis-Menten constant of 12 mM and an imax of 80 nA. Cross-talk effects on the adjacent microbands were studied and three different methods for their elimination were tested; the most efficient one involved catalase deposition on the adjacent microband. Under these conditions, the maximum response at the unmodified microbands was in the worst case about 3% compared with the response of the modified microband. This approach has the potential to fabricate a multianalyte microbiosensor.


Published in:
Analyst, 126, 7, 1068-1072
Year:
2001
ISSN:
00032654
Note:
248
Other identifiers:
Scopus: 2-s2.0-0034925465
Laboratories:




 Record created 2009-05-12, last modified 2018-03-17


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