Microfluidic Device for Nucleic Acid Fragmentation by Shear Force

A microfluidic device for random fragmentation of DNA or RNA based on the application of a hydrodynamic shear force to these biomolecules is presented. Different from conventional fragmentation instruments, the microchip-based implementation allows molecules to be repetitively sheared and broken during a single run through the device. It therefore requires comparatively few external components and has a low dead volume in the order of 0.5 μL. 48 kbp λDNA could be fragmented down to an average size of 4 kbp at a flow rate of 1 mL/min.


Editor(s):
Baba, Yoshinobu
Shoji, Shuichi
vad den Berg, Albert
Published in:
Micro Total Analysis Systems 2002, 1, 172-175
Presented at:
µTAS 2002 Symposium, Nara, Japan, 3–7 November 2002
Year:
2002
Publisher:
Berlin, Springer
Keywords:
Laboratories:




 Record created 2009-05-12, last modified 2018-01-28


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