000137589 001__ 137589
000137589 005__ 20181203021605.0
000137589 0247_ $$2doi$$a10.1021/ac0109467
000137589 022__ $$a00032700
000137589 037__ $$aARTICLE
000137589 245__ $$aAn Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases
000137589 260__ $$c2002
000137589 269__ $$a2002
000137589 336__ $$aJournal Articles
000137589 500__ $$a280
000137589 520__ $$aA new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the particles of the stationary phase are retained and stabilized. The chips were easily fabricated in poly-(dimethylsiloxane) using deep-reactive-ion-etched silicon masters, and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was packed using a vacuum with 3-μm, octadecylsilanized silica microspheres. The packing was stabilized in the column by a thermal treatment, and its stability and quality were evaluated using in-column indirect fluorescence detection. The effects of voltage on electro-osmotic flow and on efficiency were investigated, and the separation of two neutral compounds was achieved in less than 15 s.
000137589 700__ $$aCeriotti, L.
000137589 700__ $$0243301$$g104887$$ade Rooij, N. F.
000137589 700__ $$aVerpoorte, E.
000137589 773__ $$j74$$tAnalytical Chemistry$$k3$$q639-647
000137589 909C0 $$0252173$$pSAMLAB$$xU10329
000137589 909CO $$particle$$ooai:infoscience.tind.io:137589
000137589 937__ $$aSAMLAB-ARTICLE-2002-004
000137589 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000137589 980__ $$aARTICLE