000135402 001__ 135402
000135402 005__ 20180913055144.0
000135402 022__ $$a0006-291X
000135402 02470 $$2PMID$$a10548525
000135402 0247_ $$2doi$$a10.1006/bbrc.1999.1657
000135402 037__ $$aARTICLE
000135402 245__ $$aRegulation of gene expression by activation of the peroxisome proliferator-activated receptor gamma with rosiglitazone (BRL 49653) in human adipocytes
000135402 269__ $$a1999
000135402 260__ $$c1999
000135402 336__ $$aJournal Articles
000135402 520__ $$aTo better define the mechanism of action of the thiazolidinediones, we incubated freshly isolated human adipocytes with rosiglitazone and investigated the changes in mRNA expression of genes encoding key proteins of adipose tissue functions. Rosiglitazone (10(-6) M, 4 h) increased p85alphaphosphatidylinositol 3-kinase (p85alphaPI-3K) and uncoupling protein-2 mRNA levels and decreased leptin expression. The mRNA levels of insulin receptor, IRS-1, Glut 4, lipoprotein lipase, hormone-sensitive lipase, acylation-stimulating protein, fatty acid transport protein-1, angiotensinogen, plasminogen activator inhibitor-1, and PPARgamma1 and gamma2 were not modified by rosiglitazone treatment. Activation of RXR, the partner of PPARgamma, in the presence of rosiglitazone, increased further p85alphaPI-3K and UCP2 mRNA levels and produced a significant augmentation of Glut 4 expression. Because p85alphaPI-3K is a major component of insulin action, the induction of its expression might explain, at least in part, the insulin-sensitizing effect of the thiazolidinediones.
000135402 6531_ $$aMembrane Transport Proteins
000135402 6531_ $$aMitochondrial Proteins
000135402 6531_ $$aMuscle Proteins
000135402 6531_ $$aThiazolidinediones
000135402 700__ $$aRieusset, J
000135402 700__ $$0240040$$aAuwerx, J$$g185233
000135402 700__ $$aVidal, H
000135402 773__ $$j265$$k1$$q265-71$$tBiochemical and biophysical research communications
000135402 909C0 $$0252023$$pNCEM$$xU11905
000135402 909CO $$ooai:infoscience.tind.io:135402$$pSV$$particle
000135402 937__ $$aNCEM-ARTICLE-1999-004
000135402 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000135402 980__ $$aARTICLE